The training school will provide an overview of the use CRISPR/Cas9 technique as a powerful tool for gene editing. CRISPR/Cas9 technology has led to a scientific revolution in biotech. It is used for manipulating a broad range of living organisms with applications on drug discovery, research tools, cell line and animal models, agriculture and food. However there are still several limitations for therapeutic application of this technology and the greatest challenge is the safe and efficient delivery of the CRISPR-Cas9 genome-editing system to target cells in human body. Here, we will describe how delivery and on-target precision could be implemented by nanoparticle formulations. We willl concisely review recent advances in material science, synthetic biology, photonics and “Lab-on-a-Chip”systems and how this knowledge can be used to expand the range of future CRISPR/Cas9 therapeutic and biotech applications.
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